Counterimmunoelectrophoresis

Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to immunodiffusion, but with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel. The effect is rapid migration of the antibody and antigen out of their respective wells towards one another to form a line of precipitation, or a precipitin line, indicating binding.^[2]

References

^ Ling IT.; Cooksley S.; Bates PA.; Hempelmann E.; Wilson RJM. (1986). "Antibodies to the glutamate dehydrogenase of Plasmodium falciparum" (PDF). Parasitology. 92 (2): 313–324. doi:10.1017/S0031182000064088. PMID 3086819. S2CID 16953840.
^ "Counter immunoelectrophoresis".

Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. (2004). Sherris medical microbiology: an introduction to infectious diseases. New York: McGraw-Hill. Chp 15 Principles of Laboratory Diagnosis. ISBN 978-0-8385-8529-0.

External links

Counterimmunoelectrophoresis at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
https://web.archive.org/web/20070613005107/http://www.lib.mcg.edu/edu/esimmuno/ch4/electro.htm

Medical tests used in immunology (CPT 86000–86849)

Immunoprecipitation

Chromatin immunoprecipitation
Immunodiffusion
- Ouchterlony double immunodiffusion
- Radial immunodiffusion
- Immunoelectrophoresis
- Counterimmunoelectrophoresis

Immunoassay