DHX29

Protein-coding gene in the species Homo sapiens

DHX29

Identifiers

Aliases

DHX29, DDX29, DEAH-box helicase 29, DExH-box helicase 29

External IDs

OMIM: 612720; MGI: 2145374; HomoloGene: 10387; GeneCards: DHX29; OMA:DHX29 - orthologs

Gene location (Human)

Chr.	Chromosome 5 (human)^[1]

Band	5q11.2	Start	55,256,055 bp^[1]
Band	5q11.2	End	55,307,694 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 13 (mouse)^[2]

Band	13\|13 D2.2	Start	113,063,988 bp^[2]
Band	13\|13 D2.2	End	113,105,966 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

palpebral conjunctiva

secondary oocyte

visceral pleura

seminal vesicula

parietal pleura

tibia

germinal epithelium

gums

biceps brachii

gingival epithelium

Top expressed in

superior cervical ganglion

hand

tail of embryo

genital tubercle

zygote

foot

otolith organ

utricle

epiblast

oocyte

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	translation initiation factor activity ATP binding hydrolase activity nucleotide binding nucleic acid binding helicase activity ribosomal small subunit binding cadherin binding RNA binding RNA helicase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides 3'-5' RNA helicase activity
Cellular component	cytoplasm eukaryotic 43S preinitiation complex mitochondrion
Biological process	RNA processing translational initiation protein biosynthesis positive regulation of translational initiation
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


54505


218629

Ensembl


ENSG00000067248


ENSMUSG00000042426

UniProt


Q7Z478


Q6PGC1

RefSeq (mRNA)


NM_019030 NM_001345964 NM_001345965


NM_172594

RefSeq (protein)


NP_001332893 NP_001332894 NP_061903


NP_766182

Location (UCSC)

Chr 5: 55.26 – 55.31 Mb

Chr 13: 113.06 – 113.11 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

DExH-box helicase 29 (DHX29) is a 155 kDa protein that in humans is encoded by the DHX29 gene.^[5]

Function

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection.^[6] This protein may also play a role in sensing virally derived cytosolic nucleic acids.^[7] Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells.^[8]

Interactions

DHX29 has been shown to interact with the eukaryotic small ribosomal subunit (40S) and eIF3.^[9]^[10]^[11]^[12]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000067248 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000042426 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: DExH-box helicase 29".
^ Pisareva VP, Pisarev AV, Komar AA, Hellen CU, Pestova TV (December 2008). "Translation initiation on mammalian mRNAs with structured 5'UTRs requires DExH-box protein DHX29". Cell. 135 (7): 1237–50. doi:10.1016/j.cell.2008.10.037. PMC 2948571. PMID 19109895.
^ Sugimoto N, Mitoma H, Kim T, Hanabuchi S, Liu YJ (May 2014). "Helicase proteins DHX29 and RIG-I cosense cytosolic nucleic acids in the human airway system". Proceedings of the National Academy of Sciences of the United States of America. 111 (21): 7747–52. Bibcode:2014PNAS..111.7747S. doi:10.1073/pnas.1400139111. PMC 4040624. PMID 24821782.
^ Parsyan A, Shahbazian D, Martineau Y, Petroulakis E, Alain T, Larsson O, Mathonnet G, Tettweiler G, Hellen CU, Pestova TV, Svitkin YV, Sonenberg N (December 2009). "The helicase protein DHX29 promotes translation initiation, cell proliferation, and tumorigenesis". Proceedings of the National Academy of Sciences of the United States of America. 106 (52): 22217–22. Bibcode:2009PNAS..10622217P. doi:10.1073/pnas.0909773106. PMC 2799747. PMID 20018725.
^ Hashem Y, des Georges A, Dhote V, Langlois R, Liao HY, Grassucci RA, Hellen CU, Pestova TV, Frank J (May 2013). "Structure of the mammalian ribosomal 43S preinitiation complex bound to the scanning factor DHX29". Cell. 153 (5): 1108–19. doi:10.1016/j.cell.2013.04.036. PMC 3730827. PMID 23706745.
^ Hashem Y, des Georges A, Dhote V, Langlois R, Liao HY, Grassucci RA, Pestova TV, Hellen CU, Frank J (November 2013). "Hepatitis-C-virus-like internal ribosome entry sites displace eIF3 to gain access to the 40S subunit". Nature. 503 (7477): 539–43. Bibcode:2013Natur.503..539H. doi:10.1038/nature12658. PMC 4106463. PMID 24185006.
^ Pisareva VP, Pisarev AV (December 2016). "DHX29 and eIF3 cooperate in ribosomal scanning on structured mRNAs during translation initiation". RNA. 22 (12): 1859–1870. doi:10.1261/rna.057851.116. PMC 5113206. PMID 27733651.
^ des Georges A, Dhote V, Kuhn L, Hellen CU, Pestova TV, Frank J, Hashem Y (September 2015). "Structure of mammalian eIF3 in the context of the 43S preinitiation complex". Nature. 525 (7570): 491–5. Bibcode:2015Natur.525..491D. doi:10.1038/nature14891. PMC 4719162. PMID 26344199.